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KMID : 1145520150010020123
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Journal of Radiopharmaceuticals and Molecular Probes
2015 Volume.1 No. 2 p.123 ~ p.129
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Intratumoral distribution of 64Cu-ATSM and 18F-FDG in VX2 tumor xenografted rabbit
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Yoo Ran-Ji
Lee Ji-Woong
Lee Kyo-Chul
An Gwang-Il
Ko In-Ok
Chung Wee-Sup
Park Ji-Ae
Kim Kyeong-Min
Choi Yang-Kyu
Kang Joo-Hyun
Lim Sang-Moo
Lee Yong-Jin
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Abstract
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64Cu-labeled diacetyl-bis(N4-methylthiosemicarbazone) is a promising agent for internal radiation therapy and imaging of hypoxic tissues. In the study, we confirmed hypoxia regions in VX2 tumor implanted rabbits with injection 64Cu-ATSM and 18F-FDG using positron emission tomography (PET)/computed tomography (CT). PET images with 18F-FDG and 64Cu-ATSM were obtained for 40 min by dynamic scan and additional delayed PET images of 64Cu-ATSM the acquired up to 48 hours. Correlation between intratumoral O2 level and 64Cu-ATSM PET image was analyzed. 64Cu-ATSM and 18F-FDG were intravenously co-injected and the tumor was dissected and cut into slices for a dual-tracer autoradiographic analysis. In the PET imaging, 64Cu-ATSM in VX2 tumors displayed a specific uptake in hypoxic region for 48 h. The uptake pattern of 64Cu-ATSM in VX2 tumor at 24 and 48 h did not match to the 18F-FDG. Through ROI analysis, in the early
phase (dynamic scan), 18F-FDG has positive correlation with 64Cu-ATSM but late phase (24 and 48 h) of the 64Cu-ATSM showed negative correlation with 18F-FDG. High uptake of 64Cu-ATSM in hypoxic region was responded with significant decrease of oxygen pressure, which confirmed by 64Cu-ATSM PET imaging and autoradiographic analysis. In conclusion, 64Cu-ATSM can utilize for specific targeting of hypoxic region in tumor, and discrimination between necrotic- and viable hypoxic tissue.
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KEYWORD
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64Cu-ATSM, 18F-FDG, PET / CT, VX2 tumor, Hypoxia
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